Fast Raman imaging of fluids / wet in Butter

▼ Fatty ingredient and fluid distribution on the surface of butter

Fatty ingredient and fluid distribution on the surface of butter

: Fatty ingredient
: fluid
Laser wavelength : 532nm
Objective : 60x, NA=1.00
The number of spectrum : 40,000
Measurement time: 6min

 

The upper figure show Raman image of butter pinched with two glass substrates. With fast imaging, it is possible to measure fluid distribution in a sample which melts at room temperatrue without special cooling system under room temperature. Because it is not necessary of special pre-process such as staining, anybody can measure without any difficulty.

 

Confirmation of distribution of minute fluid

▼ Raman image of only fatty ingredient
Raman image of only fatty ingredient


▼ Raman image of only fluid
Raman image of only fluid


▼ Average Raman spectrum of selected area
  (Yellow:Fatty ingredient, Blue:Fluid)
Average Raman spectrum of selected area

It is easy to compare two spectrum of each selected area.

 
Left images are raman image of fatty ingredient and fluid. Though the size of some fluids is less than 1 um, it can be detected with RAMANtouch which has spatial resolution and sensitivity. By selecting any area in the images, the average spectra are shown and many analysis and process such as comparison, difference and so on can be proceeded very easily.