Ultra-fast screening by Raman imaging RAMANview


RAMANview, the wide-field Raman microscope has changed the common knowledge of space scaling of Raman imaging. A brand new world of application has been broadened.

High speed Raman imaging analysis of a tablet

RAMANview is a perfect tool for Raman imaging of tablets even with a 1 mm difference in height. Within only 14 minutes, an area of about 100 mm2 was analyzed with the 1x objective. A greater difference can be done with the 0.5x objective. Nanophoton's laser line scanning technology creates this unsurpassed speed with excellent high precision.

Measurement condition (left)
Excitation wavelength
532 nm
Objective lens
1x
Number of spectra
202 x 202 pixel
Measurement time
14 min

Measurement condition (right)
Excitation wavelength
532 nm
Objective lens
0.5x
Number of spectra
99 x 192 pixel
Measurement time
8 min

Raman imaging of the whole surface of a tablet

Acetaminophen Ethenzamide Anhydrous caffeine
Φ10.5mm ΔH1.1mm
Raman imaging of a tablet inside a blister pack

Even the imaging and analysis of a tablet inside a blister pack is possible by RAMANview. The fixation of tablet inside the pack cannot be flattened by shaving. Fixation is also impossible, so by moving the stage the tablet will swing inside the blister pack and a Raman analysis would not be possible. Only the unique technology of RAMANview can perform fast imaging under such conditions.

Excitation wavelength
532 nm
Objective lens
1x
Number of spectra
202 x 202 pixel
Measurement time
14 min

Highly precise Raman imaging analysis of tablet

High-definition Raman imaging, which is realized by point illumination with small scanning steps, is recommended for observing distribution of components precisely. The data shown on the right are the high-definition Raman images of 2 different types of antipyretic tablets. Fast laser beam scanning provides high imaging speed. The component distribution is clearly observed by the high spatial resolution over a wide range. Going even beyond, for a small amount of components, like the API's in tablets, an additional particle analysis is available. The software identify the particles one by one and a statistical analysis of diameter, area and volume is provided.

High-definition Raman images of two different tablets

Acetaminophen (AA) Ethenzamide (EZ) 
Anhydrous caffeine (AC)
Allyl isopropyl acetyl urea (AI)

AA EZ AC AI
Tablet A 52%
28%
12%
-
Tablet B
19% 48% 10% 7%
 

Particle size analysis of caffeine during formulation (comparison between pharmaceutical A and B)

Ellipticity histogram

Area histogram

Detect 1% API among the pharmaceutical formulation content

This shows the component distribution of a tablet. RAMANview's 2x objective lens is able to detect the slight amount of APIs (0.5mg) during solid dosage form by means of both a high spatial resolution and wide-area scanning.

Analysis of powder inside a plastic bag

The deep depth of focus is a great tool for bulky samples, however, even an analysis inside thin plastic bags are greatly performed with RAMANview. Raman peaks from thin film, which can be a noise for micro-Raman system, are not included in a spectrum obtained by RAMANview.

Analysis of crystallinity distribution of resin molded product

The FWHM of polyethylene terephthalate's peak at around 1720cm-1 changes due to the degree of crystallinity. The image above visualizes the difference in the degree of crystallinity from the mouth to the body part of PET bottle.

Observation of the composition distribution of Inada granite's surface

The red, blue and green parts are known as quartz, biotite and feldspar respectively from the Raman spectrum. Deterioration and erosion of the rock can be captured with a centimeter-order field-of-view by RAMANview.※This sample is provided by Professor Satoru Nakajima of Osaka University.

Observation of the distribution of SiC crystal polymorphism

This shows the distribution of polymorphic changes from 4H to 6H of a SiC crystal surface. RAMANview, equipped with wide field-of-view objective lens and fast laser beam scanning, is the best analytical tool for the analysis of the polytypic distribution over a wide range in just 10 minutes.

Crystal quality analysis of diamond

It is an analysis example of diamond file. Diamond has a sharp peak at 1330cm-1, whereas amorphous carbon which is an artificial diamond has a broad peak. It is also applicable to analysis of large-area DLC film.